Development and validation of a robust automated analysis of plasma phospholipid fatty acids for metabolic phenotyping of large epidemiological studies.
Change log
Authors
Abstract
A fully automated, high-throughput method was developed to profile the fatty acids of phospholipids from human plasma samples for application to a large epidemiological sample set (n > 25,000). We report here on the data obtained for the quality-control materials used with the first 860 batches, and the validation process used. The method consists of two robotic systems combined with gas chromatography, performing lipid extraction, phospholipid isolation, hydrolysis and derivatization to fatty-acid methyl esters, and on-line analysis. This is the first report showing that fatty-acid profiling is an achievable strategy for metabolic phenotyping in very large epidemiological and genetic studies.
Description
Keywords
0301 Analytical Chemistry, Genetics
Journal Title
Genome Med
Conference Name
Journal ISSN
1756-994X
1756-994X
1756-994X
Volume Title
Publisher
Springer Science and Business Media LLC
Publisher DOI
Sponsorship
Medical Research Council (MC_UU_12015/5)
Medical Research Council (MC_U106179471)
Medical Research Council (MC_U106179471)