XL-MS: Protein cross-linking coupled with mass spectrometry.
Change log
Authors
Holding, Andrew N
Abstract
With the continuing trend to study larger and more complex systems, the application of protein cross-linking coupled with mass spectrometry (XL-MS) provides a varied toolkit perfectly suited to achieve these goals. By freezing the transient interactions through the formation of covalent bonds, XL-MS provides a vital insight into both the structure and organization of proteins in a wide variety of conditions. This review covers some of the established methods that underpin the field alongside the more recent developments that hold promise to further realize its potential in new directions.
Description
Keywords
Cross-linking, Mass spectrometry, Proteomics, Quantitation, Structure, Systems biology, XL-MS, Animals, Cross-Linking Reagents, Humans, Mass Spectrometry, Protein Interaction Mapping, Proteins, Proteomics
Journal Title
Methods
Conference Name
Journal ISSN
1046-2023
1095-9130
1095-9130
Volume Title
89
Publisher
Elsevier BV
Publisher DOI
Sponsorship
This work was support by the Medical Research Council – United Kingdom.