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Modeling mouse and human development using organoid cultures.


Type

Article

Change log

Authors

Huch, Meritxell 
Koo, Bon-Kyoung 

Abstract

In vitro three-dimensional (3D) cultures are emerging as novel systems with which to study tissue development, organogenesis and stem cell behavior ex vivo. When grown in a 3D environment, embryonic stem cells (ESCs) self-organize into organoids and acquire the right tissue patterning to develop into several endoderm- and ectoderm-derived tissues, mimicking their in vivo counterparts. Tissue-resident adult stem cells (AdSCs) also form organoids when grown in 3D and can be propagated in vitro for long periods of time. In this Review, we discuss recent advances in the generation of pluripotent stem cell- and AdSC-derived organoids, highlighting their potential for enhancing our understanding of human development. We will also explore how this new culture system allows disease modeling and gene repair for a personalized regenerative medicine approach.

Description

Keywords

3D-Organoid Culture, Adult Stem Cell, Disease Modeling, Embryonic Stem Cell, Genetic Engineering, Animals, Cell Culture Techniques, Embryonic Development, Humans, Mice, Models, Biological, Organoids, Pluripotent Stem Cells, Regenerative Medicine

Journal Title

Development

Conference Name

Journal ISSN

0950-1991
1477-9129

Volume Title

142

Publisher

The Company of Biologists
Sponsorship
Wellcome Trust (101241/Z/13/Z)
Wellcome Trust (104151/Z/14/Z)
Wellcome Trust (097922/Z/11/B)
M.H. is a Wellcome Trust Sir Henry Dale Fellow and is jointly funded by the Wellcome Trust and the Royal Society. B.-K.K. is supported by a Sir Henry Dale Fellowship from the Wellcome Trust and the Royal Society.