Research data supporting "An orthogonal biocatalytic approach for the safe generation and use of HCN in a multi-step continuous preparation of chiral O-acetylcyanohydrins"
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Batch procedure for the CalB-catalyzed hydrolysis of ethyl cyanoformate (ECF). Flow procedure for the CalB-catalyzed hydrolysis of ethyl cyanoformate (ECF). Procedure for the immobilization of AtHNL on Celite. Procedure for the preparation of E. coli-AtHNL- and purification. Estimation of AtHNL content in lyophilized E. coli. Gene sequence for His6-AtHNL. Batch procedure for the synthesis of (R)-mandelonitrile. Synthesis of (R)-mandelonitrile by two-step (CalB-Celite-AtHNL) procedure in flow. Synthesis of (R)-mandelonitrile by two-step (CalB-E. coli-AtHNL) procedure in flow. Batch procedure for the synthesis of racemic O-acetylcyanohydrins. p. 8 S12. General flow procedure for the three-step cascade synthesis of (R)-O-acetylcyanohydrins. p. 9 S13. Enantiomeric excess (ee) determination of compounds 3a – 3f prepared by the three-step cascade flow process. p. 15 S14. Spectroscopic characterization of compound 3a – 3f. p. 16 S15 References.
