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Spatial distribution of lipid droplets during starvation: Implications for lipophagy.

Published version
Peer-reviewed

Repository DOI


Type

Article

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Authors

Barbosa, Antonio Daniel 
Siniossoglou, Symeon 

Abstract

Survival during starvation depends largely on metabolic energy, which is stored in the form of neutral lipids in specialized organelles known as lipid droplets. The precursors for the synthesis of neutral lipids are also used for membrane biogenesis, which is required for cell growth and proliferation. Therefore cells must possess mechanisms to preferentially channel lipid precursors toward either membrane synthesis or lipid droplet storage, in response to nutrient status. How this partitioning is spatially regulated within the endoplasmic reticulum (ER) where lipid droplets co-localize, remains poorly understood. We have recently shown that at the onset of starvation lipid droplets concentrate at a perinuclear ER subdomain flanking the nucleus-vacuole junction (NVJ) and that this is crucial for maintaining proper nuclear shape and ER membrane organization. Here we show that disruption of the NVJ does not block the translocation and internalization of lipid droplets into the vacuole for their degradation, which takes place at later stages of starvation. We propose that alternative pathways of lipid droplet translocation from the ER to the vacuole may exist to enable stationary phase-induced lipophagy.

Description

Keywords

lipid droplets, lipophagy, nuclear membrane, nucleus-vacuole junction

Journal Title

Communicative and Integrative Biology

Conference Name

Journal ISSN

1942-0889
1942-0889

Volume Title

9

Publisher

Taylor & Francis
Sponsorship
Medical Research Council (G0701446)
Wellcome Trust (108042/Z/15/Z)
This work was supported by the Medical Research Council (G0701446) and the Wellcome Trust (108042/Z/15/Z).