Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation.
Published version
Peer-reviewed
Repository URI
Repository DOI
Type
Article
Change log
Authors
Murphy, Annabel C
Hong, Hui
Vance, Steve
Broadhurst, R William
Leadlay, Peter F
Abstract
An in vitro model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe the apparent substrate tolerance of ketosynthase domains of the mycolactone polyketide synthase. A specific residue change was identified that led to an emphatic increase in turnover of a range of substrates.
Description
Keywords
Catalytic Domain, Mutation, Polyketide Synthases, Protein Engineering, Substrate Specificity
Journal Title
Chem Commun (Camb)
Conference Name
Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
52
Publisher
Royal Society of Chemistry (RSC)
Publisher DOI
Sponsorship
Wellcome Trust (094252/Z/10/Z)
BBSRC (BB/J007250/1)