Research data supporting "Dynamic monitoring of single cell lysis in an impedance-based microfluidic device"
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Authors
Zhou, Ying
Basu, Srinjan
Laue, Ernest D.
Seshia, Ashwin A.
Description
Real-time impedance measurements of mouse embryonic stem cells (mESCs) during the process of cell membrane lysis were performed, and the changes in the electrical properties of cells during this process were quantitatively tracked over time. The impedance spectra of individual cells over a wide frequency span was recorded over the time course. By fitting experimental data to physical models, the electrical parameters of cells were extracted and parameter variations were quantified during the process of cell membrane lysis.
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Origin, OriginPro, and Origin Viewer can be used to read .opj files. Matlab can be used to open .m and .fig files.
Keywords
microfluidics, single cell trapping, impedance spectroscopy, stem cells, real-time measurements
Publisher
University of Cambridge
Sponsorship
BBSRC [BB/K013726/1]