16S pan-bacterial PCR can accurately identify patients with ventilator-associated pneumonia
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Abstract
Ventilator-associated pneumonia (VAP) remains a challenge to intensive care units, with secure diagnosis relying on microbiological cultures that take up to 72 hours to provide a result. We sought to derive and validate a novel, real-time 16S rRNA gene PCR for rapid exclusion of VAP. Bronchoalveolar lavage (BAL) was obtained from two independent cohorts of patients with suspected VAP. Patients were recruited in a 2-centre derivation cohort and a 12-centre confirmation cohort. Confirmed VAP was defined as growth of >10
Description
Keywords
Journal Title
Conference Name
Journal ISSN
1468-3296