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Gain-of-function screen for genes that affect Drosophila muscle pattern formation.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Staudt, Nicole 
Molitor, Andreas 
Somogyi, Kalman 
Curado, Silvia 

Abstract

This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.

Description

Keywords

Animals, Body Patterning, Cell Cycle, Cell Movement, Cytoskeleton, Drosophila Proteins, Drosophila melanogaster, Gene Expression Regulation, Developmental, Genes, Insect, Genetic Techniques, Muscle Fibers, Skeletal, Muscles, Ubiquitin

Journal Title

PLoS Genet

Conference Name

Journal ISSN

1553-7404
1553-7404

Volume Title

1

Publisher

Public Library of Science (PLoS)