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Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.

Published version
Peer-reviewed

Type

Article

Change log

Authors

He, J 
Cooper, HM 
Reyes, A 
Di Re, M 
Sembongi, H 

Abstract

Mitochondrial ribosomes and translation factors co-purify with mitochondrial nucleoids of human cells, based on affinity protein purification of tagged mitochondrial DNA binding proteins. Among the most frequently identified proteins were ATAD3 and prohibitin, which have been identified previously as nucleoid components, using a variety of methods. Both proteins are demonstrated to be required for mitochondrial protein synthesis in human cultured cells, and the major binding partner of ATAD3 is the mitochondrial ribosome. Altered ATAD3 expression also perturbs mtDNA maintenance and replication. These findings suggest an intimate association between nucleoids and the machinery of protein synthesis in mitochondria. ATAD3 and prohibitin are tightly associated with the mitochondrial membranes and so we propose that they support nucleic acid complexes at the inner membrane of the mitochondrion.

Description

Keywords

ATPases Associated with Diverse Cellular Activities, Adenosine Triphosphatases, Cell Cycle Proteins, Cell Line, Tumor, DNA, Mitochondrial, HEK293 Cells, Humans, Membrane Proteins, Mitochondria, Mitochondrial Proteins, Nuclear Proteins, Nucleoproteins, Protein Biosynthesis, RNA, RNA, Messenger, RNA, Mitochondrial, Repressor Proteins, Ribosomes

Journal Title

Nucleic Acids Research

Conference Name

Journal ISSN

0305-1048
1362-4962

Volume Title

40

Publisher

Oxford University Press
Sponsorship
Medical Research Council (MC_U105663148)
Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC); Intramural program of the National Institutes of Health, National Heart, Lung and Blood Institute (to K.N.); Academy of Finland (to H.M.C.). Funding for open access charge: MRC.