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Speed limits of structured illumination microscopy

Published version
Peer-reviewed

Type

Article

Change log

Authors

Kaminski, CF 

Abstract

A theoretical framework for widefield structured illumination microscopy (SIM) reconstruction from fewer than the commonly used nine raw frame acquisitions is introduced and applied in silico and in vitro. The proposed scheme avoids the recording of redundant spatial frequency components, which was necessary in previous SIM algorithms. This allows for gentler superresolution imaging at faster speeds. A doubling of frame rates is possible solely via changes in the computational reconstruction procedure. Furthermore, we explore numerically the effect of the sample movement on the reconstruction quality and the number of raw frames recordable. Our results show that there exists a limit above which deconvolution microscopy becomes superior to SIM.

Description

Keywords

0801 Artificial Intelligence and Image Processing, Bioengineering

Journal Title

Optics Letters

Conference Name

Journal ISSN

0146-9592
1539-4794

Volume Title

42

Publisher

Optical Society of America
Sponsorship
Medical Research Council (MR/K02292X/1)
Engineering and Physical Sciences Research Council (EP/H018301/1)
Medical Research Council (MR/K015850/1)
Biotechnology and Biological Sciences Research Council (BB/H023917/1)
Medical Research Council (G0902243)
Wellcome Trust (089703/Z/09/Z)
Engineering and Physical Sciences Research Council (EPSRC) (EP/H018301/1); Medical Research Council (MRC) (MR/K015850/1, MR/K02292X/1); Wellcome Trust (089703/Z/09/Z).