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An Alternative STAT Signaling Pathway Acts in Viral Immunity in Caenorhabditis elegans.

Published version
Peer-reviewed

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Authors

Tanguy, Mélanie 
Véron, Louise 
Stempor, Przemyslaw  ORCID logo  https://orcid.org/0000-0002-9464-7475
Sarkies, Peter 

Abstract

Across metazoans, innate immunity is vital in defending organisms against viral infection. In mammals, antiviral innate immunity is orchestrated by interferon signaling, activating the STAT transcription factors downstream of the JAK kinases to induce expression of antiviral effector genes. In the nematode Caenorhabditis elegans, which lacks the interferon system, the major antiviral response so far described is RNA interference (RNAi), but whether additional gene expression responses are employed is not known. Here we show that, despite the absence of both interferon and JAK, the C. elegans STAT homolog STA-1 orchestrates antiviral immunity. Intriguingly, mutants lacking STA-1 are less permissive to antiviral infection. Using gene expression analysis and chromatin immunoprecipitation, we show that, in contrast to the mammalian pathway, STA-1 acts mostly as a transcriptional repressor. Thus, STA-1 might act to suppress a constitutive antiviral response in the absence of infection. Additionally, using a reverse genetic screen, we identify the kinase SID-3 as a new component of the response to infection, which, along with STA-1, participates in the transcriptional regulatory network of the immune response. Our work uncovers novel physiological roles for two factors in viral infection: a SID protein acting independently of RNAi and a STAT protein acting in C. elegans antiviral immunity. Together, these results illustrate the complex evolutionary trajectory displayed by innate immune signaling pathways across metazoan organisms.IMPORTANCE Since innate immunity was discovered, a diversity of pathways has arisen as powerful first-line defense mechanisms to fight viral infection. RNA interference, reported mostly in invertebrates and plants, as well as the mammalian interferon response and JAK/STAT pathway are key in RNA virus innate immunity. We studied infection by the Orsay virus in Caenorhabditis elegans, where RNAi is known to be a potent antiviral defense. We show that, in addition to its RNAi pathway, C. elegans utilizes an alternative STAT pathway to control the levels of viral infection. We identify the transcription factor STA-1 and the kinase SID-3 as two components of this response. Our study defines C. elegans as a new example of the diversity of antiviral strategies.

Description

Keywords

Caenorhabditis elegans, Orsay virus, RNA virus, STAT signaling, innate immunity, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Profiling, Immunity, Innate, Mutation, Nodaviridae, Protein-Tyrosine Kinases, RNA Interference, Signal Transduction, Trans-Activators

Journal Title

mBio

Conference Name

Journal ISSN

2161-2129
2150-7511

Volume Title

8

Publisher

American Society for Microbiology
Sponsorship
Wellcome Trust (101863/Z/13/Z)
Wellcome Trust (104640/Z/14/Z)
Cancer Research UK (18583)
European Research Council (260688)