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A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Wang, Zhengming 
Hardcastle, Thomas J 
Canto Pastor, Alex 
Yip, Wing Hin 
Tang, Shuoya 

Abstract

Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other noncanonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-dependent RNAs in a feedback loop that was disrupted by target mimic RNAs of miR6026. In lines expressing these RNAs, there was correspondingly enhanced resistance against PVX and TMV. These findings illustrate a novel miRNA pathway in plants and a crop protection strategy in which miRNA target mimicry elevates expression of defense-related mRNAs.

Description

Keywords

Dicer, RNA silencing, miRNA, tomato, virus, CRISPR-Cas Systems, Disease Susceptibility, Solanum lycopersicum, MicroRNAs, Mutation, Plant Diseases, RNA Viruses, RNA, Small Untranslated, Ribonuclease III

Journal Title

Genes Dev

Conference Name

Journal ISSN

0890-9369
1549-5477

Volume Title

32

Publisher

Cold Spring Harbor Laboratory
Sponsorship
European Research Council (340642)
International Balzan Prize Foundation (10957)
Royal Society (RP170001)
Royal Society (RP/EA/180018)