A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Abstract
Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other noncanonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-dependent RNAs in a feedback loop that was disrupted by target mimic RNAs of miR6026. In lines expressing these RNAs, there was correspondingly enhanced resistance against PVX and TMV. These findings illustrate a novel miRNA pathway in plants and a crop protection strategy in which miRNA target mimicry elevates expression of defense-related mRNAs.
Description
Keywords
Dicer, RNA silencing, miRNA, tomato, virus, CRISPR-Cas Systems, Disease Susceptibility, Solanum lycopersicum, MicroRNAs, Mutation, Plant Diseases, RNA Viruses, RNA, Small Untranslated, Ribonuclease III
Journal Title
Genes Dev
Conference Name
Journal ISSN
0890-9369
1549-5477
1549-5477
Volume Title
32
Publisher
Cold Spring Harbor Laboratory
Publisher DOI
Sponsorship
European Research Council (340642)
International Balzan Prize Foundation (10957)
Royal Society (RP170001)
Royal Society (RP/EA/180018)
International Balzan Prize Foundation (10957)
Royal Society (RP170001)
Royal Society (RP/EA/180018)