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Epistatic interactions can moderate the antigenic effect of substitutions in haemagglutinin of influenza H3N2 virus.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Koel, Björn F 
Burke, David F 
van der Vliet, Stefan 
Bestebroer, Theo M 
Rimmelzwaan, Guus F 

Abstract

We previously showed that single amino acid substitutions at seven positions in haemagglutinin determined major antigenic change of influenza H3N2 virus. Here, the impact of two such substitutions was tested in 11 representative H3 haemagglutinins to investigate context-dependence effects. The antigenic effect of substitutions introduced at haemagglutinin position 145 was fully independent of the amino acid context of the representative haemagglutinins. Antigenic change caused by substitutions introduced at haemagglutinin position 155 was variable and context-dependent. Our results suggest that epistatic interactions with contextual amino acids in the haemagglutinin can moderate the magnitude of antigenic change.

Description

Keywords

antigenic evolution, epistasis, influenza virus, Amino Acid Substitution, Antigens, Viral, Epistasis, Genetic, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Influenza A Virus, H3N2 Subtype, Influenza, Human, Mutant Proteins

Journal Title

J Gen Virol

Conference Name

Journal ISSN

0022-1317
1465-2099

Volume Title

100

Publisher

Microbiology Society

Rights

All rights reserved
Sponsorship
Office of the Director (DP1OD000490)
Human Frontier Science Program (HFSP) (RG P0050/2008-C-SMITH)
European Commission (223498)
European Commission (278976)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (#HHSN2662007000010C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (0258-0513/HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C - Option 20B)
National Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)