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A cell-engineered system to assess tumor cell sensitivity to CD8+ T cell-mediated cytotoxicity

Published version
Peer-reviewed

Type

Article

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Authors

Abstract

In vitro assays that evaluate CD8+ T cell-mediated cytotoxicity are important to aid in the development of novel therapeutic approaches to enhance anti-tumor immune responses. Here, we describe a novel cytotoxicity co-culture assay that circumvents the problem of highly variable allogeneic responses and obviates the constraints of HLA-restriction between effector and target cells. We show that this assay can be easily applied to a panel of tumor cell lines to provide additional insights into intrinsic drivers of sensitivity/resistance to T cell-mediated killing, and to evaluate the impact of targeted therapies on both tumor and T cell compartments.

Description

Keywords

T cell, cytotoxicity, immuno-oncology, immunotherapy

Journal Title

OncoImmunology

Conference Name

Journal ISSN

2162-402X
2162-402X

Volume Title

8

Publisher

Taylor & Francis
Sponsorship
Wellcome Trust (107609/Z/15/Z)
Cancer Research UK (C14303/A17197)
Maike de la Roche is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (WT107609) and Cancer Research UK (A22257).