Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR.
Accepted version
Peer-reviewed
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Repository DOI
Change log
Authors
Goldberga, Ieva https://orcid.org/0000-0003-4284-3527
Li, Rui
Chow, Wing Ying https://orcid.org/0000-0003-0719-5958
Reid, David G https://orcid.org/0000-0003-2464-5295
Bashtanova, Ulyana
Abstract
Sensitivity enhancement by isotope enrichment and DNP NMR enables detection of minor but biologically relevant species in native intact bone, including nucleic acids, choline from phospholipid headgroups, and histidinyl and hydroxylysyl groups. Labelled matrix from the aggressive osteosarcoma K7M2 cell line confirms the assignments of nucleic acid signals arising from purine, pyrimidine, ribose, and deoxyribose species. Detection of these species is an important and necessary step in elucidating the atomic level structural basis of their functions in intact tissue.
Description
Keywords
34 Chemical Sciences, Clinical Research, Rare Diseases
Journal Title
RSC Adv
Conference Name
Journal ISSN
2046-2069
2046-2069
2046-2069
Volume Title
9
Publisher
Royal Society of Chemistry (RSC)
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All rights reserved
Sponsorship
Medical Research Council (MR/M01066X/1)