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A global sampler of single particle tracking solutions for single molecule microscopy.

Accepted version
Peer-reviewed

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Authors

Wareham, Richard 
Yoon, Ji W 
Zanetti-Domingues, Laura C  ORCID logo  https://orcid.org/0000-0003-0514-3185

Abstract

The dependence on model-fitting to evaluate particle trajectories makes it difficult for single particle tracking (SPT) to resolve the heterogeneous molecular motions typical of cells. We present here a global spatiotemporal sampler for SPT solutions using a Metropolis-Hastings algorithm. The sampler does not find just the most likely solution but also assesses its likelihood and presents alternative solutions. This enables the estimation of the tracking error. Furthermore the algorithm samples the parameters that govern the tracking process and therefore does not require any tweaking by the user. We demonstrate the algorithm on synthetic and single molecule data sets. Metrics for the comparison of SPT are generalised to be applied to a SPT sampler. We illustrate using the example of the diffusion coefficient how the distribution of the tracking solutions can be propagated into a distribution of derived quantities. We also discuss the major challenges that are posed by the realisation of a SPT sampler.

Description

Keywords

Algorithms, Models, Theoretical, Motion, Single Molecule Imaging

Journal Title

PLoS One

Conference Name

Journal ISSN

1932-6203
1932-6203

Volume Title

14

Publisher

Public Library of Science (PLoS)

Rights

All rights reserved
Sponsorship
Engineering and Physical Sciences Research Council (EP/G037590/1)
BBSRC (via Rutherford Appleton Laboratory) (unknown)