Research data supporting "A Model for Determining Cardiac Mitochondrial Substrate Utilisation using Stable 13C-labelled Metabolites"
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Description
Mathematical matrix model for determining the relative oxidation of acetyl-CoA deriving from U-13C labelled sources in the Krebs cycle. Where prompted in the script, insert your measured data for the proportion of the cellular metabolite pool accounted for by each isotopologue, and press run. The model will give a readout, alpha, for the proportional contribution of U-13C substrate to Krebs cycle oxidation.
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The matrix is as described in the paper
To fit a value of alpha to experimental data, write your data values into the corresponding A, A+1, A+2.... lines and press run
Keywords
Krebs cycle, TCA Cycle, Isotopomer Analysis, Fluxomics, Metabolic Substrate Switching, LC-MS/MS, Heart
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British Heart Foundation