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Advancing Stem Cell Research through Multimodal Single Cell Analysis

Accepted version
Peer-reviewed

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Authors

Kucinski, iwo 

Abstract

Technological advances play a key role in furthering our understanding of stem cell biology, and advancing the prospects of regenerative therapies. Highly parallelized methods, developed in the last decade, can profile DNA, RNA or proteins in thousands of cells and even capture data across two or more modalities (multi-omics). This allows unbiased and precise definition of molecular cell states, thus allowing classification of cell types, tracking of differentiation trajectories and discovery of underlying mechanisms. Despite being based on destructive techniques, novel experimental and bioinformatic approaches enable embedding and extraction of temporal information, which is essential for deconvolution of complex data and establishing cause and effect relationships. Here we provide an overview of recent studies pertinent to stem cell biology, followed by an outlook on how further advances in single cell molecular profiling and computational analysis have the potential to shape the future of both basic and translational research.

Description

Keywords

Animals, Cell Differentiation, Computational Biology, Humans, Models, Biological, Muscles, Neurons, Single-Cell Analysis, Stem Cell Research, Stem Cells

Journal Title

Cold Spring Harbor Perspectives in Biology

Conference Name

Journal ISSN

1943-0264
1943-0264

Volume Title

2020

Publisher

Cold Spring Harbor Laboratory Press

Rights

All rights reserved
Sponsorship
Cancer Research UK (21762)
Wellcome Trust (206328/Z/17/Z)
Bloodwise (18002)
Wellcome Trust (079895/Z/06/Z)
Wellcome Trust (203151/Z/16/Z)
Medical Research Council (MR/S036113/1)
Medical Research Council (MC_PC_12009)
Medical Research Council (MC_PC_17230)
National Institute of Diabetes and Digestive and Kidney Diseases (R24DK106766)
Work in the Gottgens Laboratory is funded by grants from Wellcome Trust; MRC; Bloodwise; Cancer Research UK; National Institutes of Health (NIDDK DK106766); and core support grants by the Cancer Research UK Cambridge Centre and by Wellcome to the Cambridge Institute for Medical Research and Wellcome–Medical Research Council Cambridge Stem Cell Institute.