Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Hirst, Judy https://orcid.org/0000-0001-8667-6797
Kunji, Edmund https://orcid.org/0000-0002-0610-4500
Walker, John https://orcid.org/0000-0001-7929-2162
Abstract
Chorev et al. (1) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive, and lack experimental validation. The proteins are not in their ‘native’ condition: they have been stripped of tightly-bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.
Description
Keywords
Lipids, Membrane Proteins, Mass Spectrometry
Journal Title
Science (New York, N.Y.)
Conference Name
Journal ISSN
0036-8075
1095-9203
1095-9203
Volume Title
366
Publisher
Publisher DOI
Rights
All rights reserved
Sponsorship
MRC (MC_UU_00015/1)