Repository logo
 

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Accepted version
Peer-reviewed

Loading...
Thumbnail Image

Type

Article

Change log

Authors

Cagnetta, Roberta 
Shigeoka, Toshiaki 
Wunderlich, Lucia Cs  ORCID logo  https://orcid.org/0000-0001-7200-1713
Vallejo-Ramirez, Pedro  ORCID logo  https://orcid.org/0000-0002-7879-6761

Abstract

Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors associate with distinct sets of mRNAs and RNA-binding proteins. Cue stimulation of growing Xenopus retinal ganglion cell axons induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by co-exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and suggest a generalizable model for cue-selective control of the local proteome.

Description

Keywords

RNA-binding protein, axon, developmental biology, guidance receptor, human, local protein synthesis, mRNA, neuroscience, retinal ganglion cell, xenopus, Animals, Axons, Protein Biosynthesis, Proteome, RNA, Messenger, RNA-Binding Proteins, Receptors, Cell Surface, Retinal Ganglion Cells, Ribosomes, Signal Transduction, Xenopus laevis

Journal Title

Elife

Conference Name

Journal ISSN

2050-084X
2050-084X

Volume Title

8

Publisher

eLife Sciences Publications, Ltd

Rights

All rights reserved
Sponsorship
Wellcome Trust (085314/Z/08/Z)
European Research Council (322817)
Wellcome Trust (203249/Z/16/Z)
Engineering and Physical Sciences Research Council (EP/H018301/1)
Wellcome Trust (089703/Z/09/Z)
Engineering and Physical Sciences Research Council (EP/L015889/1)