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Human Germline Development from Pluripotent Stem Cells in vitro

Accepted version
Peer-reviewed

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Type

Article

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Authors

Irie, N 
Kim, S 
Surani, MA 

Abstract

© 2016 Japan Society for Ova Research.Mammalian primordial germ cells (PGCs) are specified in the early post-implantation embryo. Attempts have been made to establish in vitro PGC development since the derivation of embryonic stem cells (ESCs) from blastocysts. Despite the advances made with mouse models, similar studies in human germ cell development have not progressed because practical and ethical reasons prevent the use of early human embryos. Recently, we and others developed a robust in vitro system for producing human primordial germ cell-like cells (hPGCLCs) from ESCs and induced pluripotent stem cells (iPSCs) by inducing competency for germ cells. Strikingly, the molecular mechanism for germline differentiation is not fully conserved between mouse and human, probably because of the differences in their early embryogenesis and regulation of the pluripotent state. Here, we present a review of the current status in the field of in vitro germ cell production from pluripotent stem cells, and discuss how its usefulness could be extended to clinical applications.

Description

Keywords

3101 Biochemistry and Cell Biology, 32 Biomedical and Clinical Sciences, 31 Biological Sciences, 3206 Medical Biotechnology, 3215 Reproductive Medicine, Stem Cell Research - Induced Pluripotent Stem Cell, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research, Regenerative Medicine, Prevention, Genetics, Stem Cell Research - Embryonic - Human, Stem Cell Research - Embryonic - Non-Human, Stem Cell Research - Induced Pluripotent Stem Cell - Human, 1.1 Normal biological development and functioning, 1 Underpinning research, Generic health relevance

Journal Title

Journal of Mammalian Ova Research

Conference Name

Journal ISSN

1341-7738
1347-5878

Volume Title

33

Publisher

Japanese Society of Mammalian Ova Research

Rights

All rights reserved
Sponsorship
Wellcome Trust (096738/Z/11/Z)
Rosetrees Trust (A1178)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)