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Collagen labelling with an azide-proline chemical reporter in live cells.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Amgarten, Beatrice 
Martínez-Sáez, Nuria 
Oliveira, Bruno L 
Albuquerque, Inês S 

Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.

Description

Keywords

Alkynes, Azides, Cell Survival, Cells, Cultured, Collagen, Cyclization, Humans, Osteoblasts, Proline

Journal Title

Chem Commun (Camb)

Conference Name

Journal ISSN

1359-7345
1364-548X

Volume Title

51

Publisher

Royal Society of Chemistry (RSC)
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/G021392/1)
Engineering and Physical Sciences Research Council (EP/M003647/1)