We describe the design, construction, calibration, and characterization of a multi-primary high dynamic range (MPHDR) display system for use in vision research. The MPHDR display is the first system to our knowledge to allowfor spatially controllable, high dynamic range stimulus generation using multiple primaries.We demonstrate the high luminance, high dynamic range, and wide color gamut output of the MPHDR display. During characterization, the MPHDR display achieved a maximum luminance of 3200 cd=m2, a maximum contrast range of 3; 240; 000 V 1, and an expanded color gamut tailored to dedicated vision research tasks that spans beyond traditional sRGB displays. We discuss how the MPHDR display could be optimized for psychophysical experiments with photoreceptor isolating stimuli achieved through the method of silent substitution. We present an example case of a range of metameric pairs of melanopsin isolating stimuli across different luminance levels, from an available melanopsin contrast of117%at 75 cd=m2 to a melanopsin contrast of23%at 2000 cd=m2.