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Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development.

Published version
Peer-reviewed

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Authors

Lambooij, Jan-Paul 
Bhaskaran, Rajith 
Lancini, Cesare 
Song, Ji-Ying 

Abstract

Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9-/-animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development.

Description

Keywords

Animals, Cell Line, Cells, Cultured, Chromatin, Chromatin Assembly and Disassembly, DNA Helicases, Embryonic Development, Gene Expression Regulation, Developmental, Gene Knockout Techniques, Germ-Line Mutation, Humans, K562 Cells, Mice, Mouse Embryonic Stem Cells, Trans-Activators

Journal Title

PLoS One

Conference Name

Journal ISSN

1932-6203
1932-6203

Volume Title

15

Publisher

Public Library of Science (PLoS)
Sponsorship
KWF Kankerbestrijding (Queen Wilhelmina Research Prize)