Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Ahmed, Ibrahim
Tucci, Felicia A
Aflalo, Aure
Smith, Kenneth GC
Bashford-Rogers, Rachael JM
Abstract
The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library.
Description
Keywords
Bias, DNA, DNA Barcoding, Taxonomic, DNA Primers, Gene Library, High-Throughput Nucleotide Sequencing, Humans, Nucleic Acid Amplification Techniques, Polymerase Chain Reaction, Sequence Analysis, DNA
Journal Title
Sci Rep
Conference Name
Journal ISSN
2045-2322
2045-2322
2045-2322
Volume Title
10
Publisher
Springer Science and Business Media LLC
Publisher DOI
Sponsorship
Wellcome Trust (106068/Z/14/Z)