Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
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Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
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Keywords
Agouti Signaling Protein, Animals, Base Sequence, Cell Line, Crosses, Genetic, DNA Primers, Embryonic Stem Cells, Female, Gene Targeting, Genetic Techniques, Germ-Line Mutation, Hair Color, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Mutant Strains, Mice, Transgenic, Polymorphism, Single Nucleotide, Pregnancy, Transplantation Chimera
Journal Title
Nat Methods
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1548-7091
1548-7105
1548-7105
Volume Title
6
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Springer Science and Business Media LLC
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All rights reserved