Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Hardwick, Steven W
Luisi, Ben F
Marques, Marilis V
Abstract
The method of co-immunoprecipitation (co-IP or pulldown) enables the identification of proteins interacting in macromolecular assemblies, through the purification of a key protein by affinity chromatography using specific antibodies immobilized on a matrix. The advantages of using epitope-tagged proteins include the ability to use commercially available antibodies for affinity purifications, and typically they do not disrupt the structure of the protein complexes. Here we describe the utilization of an epitope-tagged version of Caulobacter crescentus RNase E in order to determine the composition of the RNA degradosome under different growth conditions. Several proteins that interact with the RNA degradosome were identified.
Description
Title
Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome.
Keywords
Affinity purification, Caulobacter, Epitope-tagged protein, Protein pulldown assay, RNA degradosome, RNase E, Bacterial Proteins, Caulobacter crescentus, Endoribonucleases, Multienzyme Complexes, Polyribonucleotide Nucleotidyltransferase, RNA Helicases, RNA, Bacterial
Is Part Of
RNA Remodeling Proteins
Book type
Publisher
Springer US
Publisher DOI
ISBN
978-1-0716-0934-7
Rights
All rights reserved
Sponsorship
Wellcome Trust (200873/Z/16/Z)