Two csv files (Comets_Acells.csv, Comets_Pcells.csv) containing the measurements of the angle of microtubule growth determined by tracing EB1::EGFP comets in Anterior and Posterior Compartment cells from the ventral epidermis of wildtype Drosophila third instar larvae as detailed in the article text. The first two sets of digits in each TrackID column identify the larva and cell in which the measurements were taken. A csv file (Ds-EGFP_wt.csv) containing normalized peak intensities of Ds::EGFP at anterior and posterior cell boundaries of the ventral epidermis of wildtype Drosophila third instar larvae as detailed in the article text. A similar file (Ds-EGFP_ectoDs.csv) with data of larvae overexpressing ectoDs in tendon cells.