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Trophectoderm mechanics direct epiblast shape upon embryo implantation.

Accepted version
Peer-reviewed

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Authors

Zernicka-Goetz, Magdalena  ORCID logo  https://orcid.org/0000-0002-7004-2471

Abstract

Implantation is a hallmark of mammalian embryogenesis during which embryos establish their contacts with the maternal endometrium, remodel, and undertake growth and differentiation. The mechanisms and sequence of events through which embryos change their shape during this transition are largely unexplored. Here, we show that the first extraembryonic lineage, the polar trophectoderm, is the key regulator for remodeling the embryonic epiblast. Loss of its function after immuno-surgery or inhibitor treatments prevents the epiblast shape transitions. In the mouse, the polar trophectoderm exerts physical force upon the epiblast, causing it to transform from an oval into a cup shape. In human embryos, the polar trophectoderm behaves in the opposite manner, exerting a stretching force. By mimicking this stretching behavior in mouse embryogenesis, we could direct the epiblast to adopt the disc-like shape characteristic of human embryos at this stage. Thus, the polar trophectoderm acts as a conserved regulator of epiblast shape.

Description

Keywords

epiblast, morphogenesis, mouse/human implantation, tissue remodeling, trophectoderm, Animals, Cell Differentiation, Embryo Implantation, Embryonic Development, Germ Layers, Humans, Mice

Journal Title

Cell Rep

Conference Name

Journal ISSN

2211-1247
2211-1247

Volume Title

34

Publisher

Elsevier BV

Rights

All rights reserved
Sponsorship
European Research Council (669198)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (721537)
Wellcome Trust (207415/Z/17/Z)
European Research Council (RG77946), the Welcome Trust (207415/Z/17/Z), the EU Horizon 2020 Marie Sklodowska-Curie actions (ImageInLife,721537) and Open Philanthropy and Weston Haven grants at Caltech.