Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Couturier, Maxime
Bhalara, Hiral D
Monson, Rita E
Salmond, George PC
Leeper, Finian J https://orcid.org/0000-0003-3408-5199
Abstract
A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathway is an intermediate. In addition, the enzyme PigE was originally described as a transaminase but we present evidence that it also catalyses the reduction of the thioester intermediate to its aldehyde substrate.
Description
Keywords
3404 Medicinal and Biomolecular Chemistry, 34 Chemical Sciences, Infection
Journal Title
RSC Chem Biol
Conference Name
Journal ISSN
2633-0679
2633-0679
2633-0679
Volume Title
2
Publisher
Royal Society of Chemistry (RSC)
Publisher DOI
Sponsorship
Biotechnology and Biological Sciences Research Council (1645655)
Biotechnology and Biological Sciences Research Council (BB/K001833/1)
Biotechnology and Biological Sciences Research Council (BB/N008081/1)
Biotechnology and Biological Sciences Research Council (BB/K001833/1)
Biotechnology and Biological Sciences Research Council (BB/N008081/1)
BBSRC, UK, awards BB/N008081/1 and BB/K001833/1
Frances and Augustus Newman foundation
Cambridge Commonwealth Trust
Emmanuel College, Cambridge