Research data supporting "Investigation of conventional and unconventional protein secretion using galectin-3"
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Dataset
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Description
These data files support Stephanie Popa's thesis submitted for the degree of Doctor of Philosophy.
A1: Raw sgRNA count for GeCKOv2 screen (Stewart et al., 2017), .txt file. A2: MaGECK-MLE output for GeCKOv2 screen, .txt file. A3: Raw sgRNA count for Brunello screen, .txt file. A4: MaGECK output for Brunello screen, .xlsx file. A5: siGenome smart pool plate layout, .xlsx file. A6: Luminescence values generated from ss-HRP screen, .txt file. A7: Unprocessed image files generated from Golgi morphology screen, zipped folders (.zip) containing .tif files. A7 has been divided into three folders due to large size (A7-1, A7-2, A7-3).
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Software / Usage instructions
MaGECK was used to create A2 and A4: https://sourceforge.net/p/mageck/wiki/Home/
Keywords
CRISPR, CRISPR screen, MaGECK, Galectin-3, Unconventional protein secretion, Protein secretion, Conventional protein secretion, Glycoprotein secretion, siRNA screen, Galectins, GPR161, TMEM220, MARCH2, TMEM199, COL14A1, MXRA7, FAM98B, FAM102B
Publisher
Sponsorship
Wellcome Trust PhD studentship (109152)