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Current Methods and Pipelines for Image-Based Quantitation of Nuclear Shape and Nuclear Envelope Abnormalities.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Janssen, Anne FJ 
Breusegem, Sophia Y 

Abstract

Any given cell type has an associated "normal" nuclear morphology, which is important to maintain proper cellular functioning and safeguard genomic integrity. Deviations from this can be indicative of diseases such as cancer or premature aging syndrome. To accurately assess nuclear abnormalities, it is important to use quantitative measures of nuclear morphology. Here, we give an overview of several nuclear abnormalities, including micronuclei, nuclear envelope invaginations, blebs and ruptures, and review the current methods used for image-based quantification of these abnormalities. We discuss several parameters that can be used to quantify nuclear shape and compare their outputs using example images. In addition, we present new pipelines for quantitative analysis of nuclear blebs and invaginations. Quantitative analyses of nuclear aberrations and shape will be important in a wide range of applications, from assessments of cancer cell anomalies to studies of nucleus deformability under mechanical or other types of stress.

Description

Keywords

lamina, nuclear envelope, nuclear envelope abnormality, nuclear morphology, Humans, Cell Nucleus, Neoplasms, Nuclear Envelope

Journal Title

Cells

Conference Name

Journal ISSN

2073-4409
2073-4409

Volume Title

11

Publisher

MDPI AG
Sponsorship
Wellcome Trust (206242/Z/17/Z)
Federation of the European Biochemical Societies (FEBS) (Anne Janssen FEBS LTF)
FEBS Long-Term Fellowship.