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Research data supporting "Investigating the role of the circadian clock in the physiology of wheat".


Change log

Authors

Taylor, Laura 
Hannah, Matthew 

Description

Electronic Tables: In Table 1 the circadian clock gene ID's previously determined using the wheat genome assembly TGAC (Wittern, L. M., 2019, https://doi.org/10.17863/CAM.26460) are listed followed by updated IWGSC RefSeq v1.1 gene identifiers. Table 2-4 detail wheat orthologs to Arabidopsis circadian clock genes as determined by Plaza v5, Plaza v6 and Ensembl Compara respectively. In Table 2 and 3 the Evidence(s) column refers to the different method used by Plaza to determine if a gene is an ortholog. A 'Y" letter (i.e. YES) indicates that a gene was identified as an ortholog by that particular method.
Table 5 outlines the results of analysis using GMAP. The IWGSC RefSeq v1.1 circadian clock gene sequences were mapped to the genome sequences of different wheat cultivars (row 1) and summary statistics were calculated.

Sequence alignments: Sequence alignments for circadian clock genes LUX, ELF3, GI and LHY were created between IWGS Refseqv1.1 and wheat cultivars important in modern breeding efforts. Each alignment is preceded by a sequence overview. The height of the pink bar graphically represents the level of conservation between the sequences.

For more information see methods section of Chapter 3 of the associated manuscript.

Version

Software / Usage instructions

Ensembl ID History Converter Ensembl compara Plaza V5, V6 (https://bioinformatics.psb.ugent.be/plaza/) GMAP (Citation, GMAP: a genomic mapping and alignment program for mRNA and EST sequences, Thomas D. Wu, Colin K. Watanabe Bioinformatics, Volume 21, Issue 9, , Pages 1859–1875, https://doi.org/10.1093/bioinformatics/bti310)

Keywords

Circadian clock, Plants

Publisher

Sponsorship
BBSRC (1947959)
Biotechnology and Biological Sciences Research Council (1947959)
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