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A Platform Approach to Protein Encapsulates with Controllable Surface Chemistry.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Osojnik Črnivec, Ilja Gasan  ORCID logo  https://orcid.org/0000-0002-7275-8705
Scherman, Oren A 

Abstract

The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient's preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (Dh) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of Dh is improved when measured at the spray dryer outlet temperature compared to room temperature (R2 = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.

Description

Keywords

EISA, core-shell, encapsulation, excipient, formulation, protein, spray dry, Emulsions, Particle Size, Powders, Proteins, Solvents, Temperature, Water

Journal Title

Molecules

Conference Name

Journal ISSN

1420-3049
1420-3049

Volume Title

Publisher

MDPI AG