ࡱ > [ ] X Y Z bjbjT~T~ . 6 6 | | 0 $ G t 2 2 2 j 2 2 2 2 PfE@ 0 ^ 2 0 2 ] X ] 2 ] 2 P 2 L ] | : Additional data file 2. Analysis workflow for the Burkholderia pan genome array (BPGA). Additional data file 2. Analysis workflow for the Burkholderia pan genome array (BPGA). Genome sequences were processed using three independent species-specific pipelines (Bp, Bt and Bc). For the Bp genomes, each Bp strain genome was aligned, first against the BpK96243 reference genome, and subsequently against an expanding Bp pan genome, to identify novel sequences. A similar pipeline was used to define the Bt pan genome. For Bc, we identified genes in BcJ2315 not found in BpK96243 by a reciprocal Blast protocol (3019 Bc-specific genes). These Bc specific genes were also added to the pan genome array. The final composition of BPGA used for array construction (total length = 22.3 Mb) comprises of 12.34 Mb (55%) of non-redundant sequence from 23 Bp strains (BpK96243 and 22 additional Bp genomes, red bar). The Bt pan-genome comprises 7.35 Mb (34%) of non-redundant sequence from 4 Bt strains (Bt E264 and 3 additional Bt genomes, blue bar). The array also contains 2.64 Mb (11%) of additional Bc-specific sequence found in Bc J2315 (green) but not Bp K96243. See Main Text for details on array construction. 2 > W X Y Z q B a < = \ ︨whwhwhwU %h 2 h B*CJ OJ QJ aJ ph h 2 hV CJ OJ QJ aJ h 2 h CJ OJ QJ aJ "h 2 h 56CJ OJ QJ aJ h 2 h 5CJ OJ QJ aJ h 2 h6 5CJ OJ QJ aJ (j h 2 h6 5CJ OJ QJ UaJ "h 2 h6 56CJ OJ QJ aJ h 2 h6 5CJ OJ QJ aJ h 2 hLA 5CJ OJ QJ aJ X Z $a$gdG , 1h/ =!"#$% _ D d =+( > C A b v^ TIѿ`X R^ D nJ^ TIѿ`XPNG IHDR ' sRGB pHYs j IDATx^]%$X^ݡ8-P\)ҏR(P[R\F fv7o˕#gΜ9gf2l֥!@J)R H)P|()"%J)R @J)R x 0%僔)R H)RH)"mX)R H)R @ SRH)R @J))L)҆IR @J)R 0%偔)R H)RH)"mX)R H)R @ SRH)R @J))L)҆IR @J)R d.} )#H)R @J"@&)P^S|ӐiIR H)R @WP ]@II^$t$L.]0i)R H)R`@K$_M );FiUN)R @J.@KDϥ_-KHM!\^vRnHO)R @JC GC%\i\tۥ&b)B$#&xZ1-IJ)R (P]]M}KKKcµ4+2N)%[O (eee}]wꫯԘ$I&R @J)f@ (FPcʔ) SESdX1m ?<ԙs9<92rJ)R ȧH&MO?ᦛnqgB*1^ PQX"2p~{E:tC= +lR @J)R t?Py䑗_~vmw뭷N< Ő?unzȸCSFJ+=s[4)R H)RxꩧXcSN9O8q"\"J;Ln`>|.oN:tLH)R @J]A.oGH3t/mC'Pt^!zԨQ>o~ tfJ)R H) KvioCb:kTaG\_-?H*(mg9@J)R t5:[n\jCto'0E5Q Ro1dȐu~J)R H)& ,r" YB]X۔Z{pr^1+++;iR)R H)R @)Q%/Zkjzn%E>\wʨR @J)R 6XK~:D=Srjh`[iӐR @J)R !N*-jOپ)RJ'j:jij)R H)R`as06R'a Ee')|S:QyJ)R H)bWc0Ev,1ٺ䧒DJ)R H)P6E}bY)REMԼjX*@J)R j +EK^btU)VGQsFE4)R H)R@f[],"LQf4HRJ)R @J):S~oډ0LR1=m++&R @J)R t*ݮS+0LI:1wڎ1M!@J)R {]bab늺uEiH)R @J)Td 0LZI*KL)R @J32gAnÔkoZk쫯]rL_~R;^G`뭷fmj+.qO?-첷~{~"?K.yM7#}pzG[fev,okǷ-}G;VZ颋.jxm}{oJw}Ygs9=PgdSvVJ^}'sMSk3dmKp '!O<~`O}ݗ_~/r_۰a009Mz'L@k) K/S .bmvmfoKj>|CU2dHSVxSЫ~_{|k5?HGۛo6LNo7lvʇ.鷒N;?>]~/ƝwI>lǓSQ["Lꫯ8tz>/*L8qʔ)@FLImJf~xCp@<SHg [wQʝ^I +z̉':@ @0 e/W]ugqFp\#ۑ<ʷyK#W^?amƘe;5:]Zf(t|^YdThߏ?(όt9}Oyq~$>4hO߹Ԭ.;= STU1ŞwJ%D %:Wk#`PG:>GEUU@43WAJ4~XG_rW͆?4IYx7H82>S Ǐ#'T?oI]sox.g!f߾{+S`&RSYR)MPx!gf?$ 4'k1)/p-#־&Mb#Dl@ ߈hW^y,b()3 pIX~eyȫq\s'DӂA@sҔ(/pf7izK/bvo6ΚM;Ubb{JH\,ֲј?XN~ZQQ()ЩO蒭T7雭taR?8ԋYmĈkܸqzDЭuNtb}Z\ġFB&Kv0l6?B})h]xBiPO^z'5_ɖmw-b-:;<_%+O'1 eq|ͧ(UW^-N?tYd<x!y^I qBl)` ̾R!1+TjX wߝ8[!ʠo]Vdcƌ̉B\rCj`G+p~-PFzFA=yzSA+j)mc*\ |Lj^oiaYrA$lͦ'xbm6s0TP`e?_c5bV*oD8D^ziK$qqq P=C=wyB3a AJoZm6w+h{+0̚;#82?O}(6pU 65C vM:=>뮻x@ЭagчIᅬ`Vgt Mo=`)L \0qA`2g7'Y)gi&vX'r32Ba&7FTBi'H[|yfnHͯ:c2i RR0O&nQ*sA: bn~<%Vs8$YphַJY&p:tEƮGD"% R5JdI1H{\3pM2Њ+tPF6;M)$#~n<ԑYeÚ*@I"ycǎU|9'1
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