This Readme.txt file was generated on 20200720 by LINA HACKER.

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GENERAL INFORMATION
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Title of Dataset: Photoacoustic imaging resolves species-specific differences in haemoglobin concentration and oxygenation

Author Information

	Principal Investigator: Sarah E. Bohndiek, Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, United Kingdom, seb53@cam.ac.uk;
	Co-Principal Investigator: Isabel Quiros-Gonzalez, Morphology and Cell Biology Department, School of Medicine, IUOPA and FINBA. University of Oviedo, Julian Claveria 33006, Spain, Isabel.QuirosGonzalez@cruk.cam.ac.uk
	Associate or Co-investigator: Lina Hacker, Department of Physics, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, United Kingdom, lh592@cam.ac.uk
	Alternate Contact: Joanna Brunker, Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge, CB2 0RE, U.K.
	Alternate Contact: Ewan St. John Smith, Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD, U.K.


Date of data collection: 20170403 to 20191015

Geographic location of data collection: Cambridge, Cambridgeshire, United Kingdom 

Information about funding sources or sponsorship that supported the collection of the data:

This work was funded by Cancer Research UK under grant numbers C14303/A17197, C9545/A29580, C47594/A16267 and C197/A16465 (SEB) as well as C56829/A22053 (EStJS). LH is funded by a studentship from the National Physical Laboratory.
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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse:
CC BY

Recommended citation for the data:

Citation for and links to publications that cite or use the data:

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DATA & FILE OVERVIEW
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Folders:

Figure 1:
Raw data acquired with a Multispectral Optoacoustic Tomography (MSOT) system (iThera Medical) and a haematology analyser (Mythic 18 Vet, Woodley Equipment).
Data was analysed and plotted using PRISM.
File formats: Excel files (.xlsx).

Figure 2: 
Raw data acquired with a Multispectral Optoacoustic Tomography (MSOT) system (iThera Medical) and a haematology analyser (Mythic 18 Vet, Woodley Equipment).
Data was analysed and plotted using PRISM.
File formats: Excel files (.xlsx).

Figure 3:
Multispectral Optoacoustic Tomography (MSOT) raw data (iThera Medical). 
MATLAB file containing calcuated MSOT sO2 values (sO2 = HbO/(HbO+HbR)) and aligned pO2 values acquired in parallel using  an oxygen fluorescence quenching needle probes (Oxford Optronix, NX-BF/O/E).
MATLAB files containing literature spectra for rat, mouse, and naked mole-rat (taken from Schmidt-Neilsen, K. & Larimer, J. L. Oxygen Dissociation Curves of Mammalian Blood in Relation To Body Size and Johansen, K., Lykkeboe, G., Weber, R. E. & Maloiy, G. M. O. M. O. Blood respiratory properties in the naked mole rat Heterocephalus glaber, a mammal of low body temperature. Respir. Physiol. 28, 303–314 (1976)).
MATLAB code for plotting the data.
File formats: Excel files (.xlsx) and MATLAB R2016b (.m and .mat) files

Figure 4:
Multispectral Optoacoustic Tomography (MSOT) raw data (iThera Medical).
Jupyter Notebook file for analysing and plotting the data. Data was plotted using PRISM.
File formats: Excel files (.xlsx) and Jupyter Notebook (.ipynb) file.

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METHODOLOGICAL INFORMATION
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Please refer to main publication for more details on data acquisition.

Methods for processing the data:
Data has been analysed using PRSIM, MATLAB R2016B and Jupyter Notebook.


People involved with sample collection, processing, analysis and/or submission: 
L.H. assembled, analysed and interpreted the data and wrote the manuscript. I.Q.G. and S.E.B conceived and designed the study. J.B. assisted with the data acquisition and analysis. E.J.S. provided the naked-mole rat blood. S.E.B.,E.J.S, J.B. and I.Q.G.assisted in writing the manuscript.