Now showing items 1-9 of 9

    • Breaking the waves: improved detection of copy number variation from microarray-based comparative genomic hybridization 

      Marioni, John C.; Thorne, Natalie P.; Valsesia, Armand; Fitzgerald, Tomas; Redon, Richard; Fiegler, Heike; Andrews, T. Daniel et al. (2007-10-25)
      Abstract Background Large-scale high throughput studies using microarray technology have established that copy number variation (CNV) throughout the genome is more frequent than previously thought. Such variation is known ...
    • Characterisation of microRNA expression in post-natal mouse mammary gland development 

      Avril-Sassen, Stefanie; Goldstein, Leonard D.; Stingl, John; Blenkiron, Cherie; Le Quesne, John; Spiteri, Inmaculada; Karagavriilidou, Konstantina et al. (2009-11-20)
      Abstract Background The differential expression pattern of microRNAs (miRNAs) during mammary gland development might provide insights into their role in regulating the homeostasis of the mammary epithelium. Our aim was to ...
    • Data analysis issues for allele-specific expression using Illumina's GoldenGate assay 

      Ritchie, Matthew E.; Forrest, Matthew S.; Dimas, Antigone S.; Daelemans, Caroline; Dermitzakis, Emmanouil T.; Deloukas, Panagiotis; Tavare, Simon (2010-05-26)
      Abstract Background High-throughput measurement of allele-specific expression (ASE) is a relatively new and exciting application area for array-based technologies. In this paper, we explore several data sets which make use ...
    • High-resolution aCGH and expression profiling identifies a novel genomic subtype of ER negative breast cancer 

      Chin, Suet-Feung; Teschendorff, Andrew E.; Marioni, John C.; Wang, Yanzhong; Barbosa-Morais, Nuno L.; Thorne, Natalie P.; Costa, Jose L. et al. (2007-10-07)
      Abstract Background The characterization of copy number alteration patterns in breast cancer requires high-resolution genome-wide profiling of a large panel of tumor specimens. To date, most genome-wide array comparative ...
    • High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta 

      Daelemans, Caroline; Ritchie, Matthew E.; Smits, Guillaume; Abu-Amero, Sayeda; Sudbery, Ian M.; Forrest, Matthew S.; Campino, Susana et al. (2010-04-19)
      Abstract Background Imprinted genes show expression from one parental allele only and are important for development and behaviour. This extreme mode of allelic imbalance has been described for approximately 56 human genes. ...
    • MicroRNA expression profiling of human breast cancer identifies new markers of tumour subtype 

      Blenkiron, Cherie; Goldstein, Leonard D.; Thorne, Natalie P.; Spiteri, Inmaculada; Chin, Suet-Feung; Dunning, Mark J.; Barbosa-Morais, Nuno L. et al. (2007-10-08)
      Abstract Background MicroRNAs (miRNAs), a class of short non-coding RNAs found in many plants and animals, often act post-transcriptionally to inhibit gene expression. Results Here we report the analysis of miRNA expression ...
    • The cost of reducing starting RNA quantity for Illumina BeadArrays: A bead-level dilution experiment. 

      Lynch, Andrew G.; Hadfield, James; Dunning, Mark J.; Osborne, Michelle; Thorne, Natalie P.; Tavare, Simon (2010-10-06)
      Abstract Background The demands of microarray expression technologies for quantities of RNA place a limit on the questions they can address. As a consequence, the RNA requirements have reduced over time as technologies ...
    • Transcriptional profiling of MnSOD-mediated lifespan extension in Drosophila reveals a species-general network of aging and metabolic genes 

      Curtis, Christina; Landis, Gary N.; Folk, Donna; Wehr, Nancy B.; Hoe, Nicholas; Waskar, Morris; Abdueva, Diana et al. (2007-12-09)
      Abstract Background Several interventions increase lifespan in model organisms, including reduced insulin/insulin-like growth factor-like signaling (IIS), FOXO transcription factor activation, dietary restriction, and ...
    • Using expression arrays for copy number detection: an example from E. Coli 

      Skvortsov, Dmitriy; Abdueva, Diana; Stitzer, Michael E.; Finkel, Steven E.; Tavare, Simon (2007-06-14)
      Abstract Background The sequencing of many genomes and tiling arrays consisting of millions of DNA segments spanning entire genomes have made high-resolution copy number analysis possible. Microarray-based comparative ...