Now showing items 1-5 of 5

    • Breaking the waves: improved detection of copy number variation from microarray-based comparative genomic hybridization 

      Marioni, John Carlo; Thorne, Natalie P; Valsesia, Armand; Fitzgerald, Tomas; Redon, Richard; Fiegler, Heike; Andrews, T Daniel et al. (2007-10-25)
      Abstract Background Large-scale high throughput studies using microarray technology have established that copy number variation (CNV) throughout the genome is more frequent than previously thought. Such variation is known ...
    • The cost of reducing starting RNA quantity for Illumina BeadArrays: A bead-level dilution experiment. 

      Lynch, Andrew Graeme; Hadfield, James; Dunning, Mark James; Osborne, Michelle; Thorne, Natalie P; Tavare, Simon (2010-10-06)
      Abstract Background The demands of microarray expression technologies for quantities of RNA place a limit on the questions they can address. As a consequence, the RNA requirements have reduced over time as technologies ...
    • High-resolution aCGH and expression profiling identifies a novel genomic subtype of ER negative breast cancer 

      Chin, Suet Feung; Teschendorff, Andrew E; Marioni, John Carlo; Wang, Yanzhong; Barbosa-Morais, Nuno Luis; Thorne, Natalie P; Costa, Jose L et al. (2007-10-07)
      Abstract Background The characterization of copy number alteration patterns in breast cancer requires high-resolution genome-wide profiling of a large panel of tumor specimens. To date, most genome-wide array comparative ...
    • MicroRNA expression profiling of human breast cancer identifies new markers of tumour subtype 

      Blenkiron, Cherie; Goldstein, Leonard David; Thorne, Natalie P; Spiteri, Inmaculada; Chin, Suet Feung; Dunning, Mark James; Barbosa-Morais, Nuno Luis et al. (2007-10-08)
      Abstract Background MicroRNAs (miRNAs), a class of short non-coding RNAs found in many plants and animals, often act post-transcriptionally to inhibit gene expression. Results Here we report the analysis of miRNA expression ...
    • Missing channels in two-colour microarray experiments: Combining single-channel and two-channel data 

      Lynch, Andrew Graeme; Neal, David Edgar; Kelly, John D; Burtt, Glynn; Thorne, Natalie P (2007-01-25)
      Abstract Background There are mechanisms, notably ozone degradation, that can damage a single channel of two-channel microarray experiments. Resulting analyses therefore often choose between the unacceptable inclusion of ...