Now showing items 1-6 of 6

    • ALS/FTD mutation-induced phase transition of FUS liquid droplets and reversible hydrogels into irreversible hydrogels impairs RNP granule function 

      Murakami, Tetsuro; Qamar, Seema; Lin, Julie Qiaojin; Kaminski, Gabriele Suzanne; Rees, Eric John; Miyashita, Akinori; Costa, Ana R et al. (Elsevier, 2015-10-29)
      The mechanisms by which mutations in FUS and other RNA binding proteins cause ALS and FTD remain controversial. We propose a model in which the low complexity (LC) domains of FUS drive its physiologically reversible assembly ...
    • Phase Separation of FUS is Modulated by Methylation State of Cation-π Interactions and Interaction with TNPO1 

      St George-Hyslop, Peter Henry; Qamar, Seema; Wang, Guozhen; Randle, SJ; Ruggeri, Francesco Simone; Varela, Juan Alberto; Kaminski, Clemens Friedrich et al.
      Reversible phase separation, which underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles, is in part driven by the intrinsically disordered low complexity (LC) domain of FUS. Here, we ...
    • Single-molecule Imaging Reveals that Small Amyloid-β1-42 Oligomers Interact with the Cellular Prion Protein (PrPC) 

      Ganzinger, Kristina A; Narayan, Priyanka; Qamar, Seema; Weimann, Laura; Ranasinghe, Rohan Tissa; Aguzzi, Adriano; Dobson, Christopher Martin et al. (Wiley, 2014-10-07)
      Oligomers of the amyloid-β peptide (Aβ) play a central role in the pathogenesis of Alzheimer’s disease and have been suggested to induce neurotoxicity by binding to a plethora of cell-surface receptors. However, the ...
    • The topology, structure and PE interaction of LITAF underpin a Charcot-Marie-Tooth disease type 1C 

      Ho, AK; Wagstaff, JL; Manna, Paul Thomas; Wartosch, Lena; Qamar, Seema; Garman, EF; Freund, SMV et al. (BioMed Central, 2016-12-07)
      BACKGROUND: Mutations in Lipopolysaccharide-induced tumour necrosis factor-α factor (LITAF) cause the autosomal dominant inherited peripheral neuropathy, Charcot-Marie-Tooth disease type 1C (CMT1C). LITAF encodes a 17 kDa ...
    • TREM2 shedding by cleavage at the H157-S158 bond is accelerated for the Alzheimer’s disease-associated H157Y variant 

      Thornton, P; Sevalle, J; Deery, Michael John; Fraser, G; Zhou, Y; Ståhl, S; Franssen, EH et al. (Wiley, 2017-09-01)
      We have characterised the proteolytic cleavage events responsible for the shedding of Triggering Receptor Expressed on Myeloid cells 2 (TREM2) from primary cultures of human macrophages, murine microglia and TREM2-expressing ...
    • Vigilin interacts with Signal Peptide Peptidase 

      Lu, Stephen Hsueh-Jeng; Jeon, Amy Hye Won; Schmitt-Ulms, Gerold; Qamar, Seema; Dodd, Roger Bradley; McDonald, Beth; Li, Yi et al. (2012-05-18)
      AbstractBackgroundSignal peptide peptidase (SPP), a member of the presenilin-like intra-membrane cleaving aspartyl protease family, migrates on Blue Native (BN) gels as 100 kDa, 200 kDa and 450 kDa species. SPP has recently ...