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dc.contributor.authorChaudhuri, Royen
dc.contributor.authorAllen, Andrew Gen
dc.contributor.authorOwen, Paul Jen
dc.contributor.authorShalom, Gilen
dc.contributor.authorStone, Karlen
dc.contributor.authorHarrison, Marcusen
dc.contributor.authorBurgis, Timothy Aen
dc.contributor.authorLockyer, Michaelen
dc.contributor.authorGarcia-Lara, Jorgeen
dc.contributor.authorFoster, Simon Jen
dc.contributor.authorPleasance, Stephen Jen
dc.contributor.authorMaskell, Sarahen
dc.contributor.authorMaskell, Duncanen
dc.contributor.authorCharles, Ian Gen
dc.date.accessioned2011-06-13T15:29:27Z
dc.date.available2011-06-13T15:29:27Z
dc.date.issued2009-07-01en
dc.identifier.citationBMC Genomics 2009, 10:291
dc.identifier.issn1471-2164
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237575
dc.description.abstractAbstract Background In recent years there has been an increasing problem with Staphylococcus aureus strains that are resistant to treatment with existing antibiotics. An important starting point for the development of new antimicrobial drugs is the identification of "essential" genes that are important for bacterial survival and growth. Results We have developed a robust microarray and PCR-based method, Transposon-Mediated Differential Hybridisation (TMDH), that uses novel bioinformatics to identify transposon inserts in genome-wide libraries. Following a microarray-based screen, genes lacking transposon inserts are re-tested using a PCR and sequencing-based approach. We carried out a TMDH analysis of the S. aureus genome using a large random mariner transposon library of around a million mutants, and identified a total of 351 S. aureus genes important for survival and growth in culture. A comparison with the essential gene list experimentally derived for Bacillus subtilis highlighted interesting differences in both pathways and individual genes. Conclusion We have determined the first comprehensive list of S. aureus essential genes. This should act as a useful starting point for the identification of potential targets for novel antimicrobial compounds. The TMDH methodology we have developed is generic and could be applied to identify essential genes in other bacterial pathogens.
dc.languageEnglishen
dc.language.isoen
dc.titleComprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH)en
dc.typeArticle
dc.date.updated2011-06-13T15:29:27Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderChaudhuri et al.; licensee BioMed Central Ltd.
prism.publicationDate2009en
dcterms.dateAccepted2009-07-01en
rioxxterms.versionofrecord10.1186/1471-2164-10-291en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2009-07-01en
dc.contributor.orcidMaskell, Duncan [0000-0002-5065-653X]
dc.identifier.eissn1471-2164
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (APG19115)
pubs.funder-project-idBBSRC (BB/D017947/1)


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