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dc.contributor.authorYang, Jennieen
dc.contributor.authorDownes, Kateen
dc.contributor.authorHowson, Joannaen
dc.contributor.authorNutland, Sarahen
dc.contributor.authorStevens, Helenen
dc.contributor.authorWalker, Neil Men
dc.contributor.authorTodd, Johnen
dc.date.accessioned2011-06-14T10:03:10Z
dc.date.available2011-06-14T10:03:10Z
dc.date.issued2011-04-27en
dc.identifier.citationBMC Medical Genetics 2011, 12:59
dc.identifier.issn1471-2350
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237586
dc.description.abstractAbstract Background Linkage and congenic strain analyses using the nonobese diabetic (NOD) mouse as a model for human type 1 autoimmune diabetes (T1D) have identified several NOD mouse Idd (insulin dependent diabetes) loci, including Slc11a1 (formerly known as Nramp1). Genetic variants in the orthologous region encompassing SLC11A1 in human chromosome 2q35 have been reported to be associated with various immune-related diseases including T1D. Here, we have conducted association analysis of this candidate gene region, and then investigated potential correlations between the most T1D-associated variant and RNA expression of the SLC11A1 gene and its splice isoform. Methods Nine SNPs (rs2276631, rs2279015, rs1809231, rs1059823, rs17235409 (D543N), rs17235416 (3'UTR), rs3731865 (INT4), rs7573065 (-237 C→T) and rs4674297) were genotyped using TaqMan genotyping assays and the polymorphic promoter microsatellite (GT)n was genotyped using PCR and fragment length analysis. A maximum of 8,863 T1D British cases and 10,841 British controls, all of white European descent, were used to test association using logistic regression. A maximum of 5,696 T1D families were also tested for association using the transmission/disequilibrium test (TDT). We considered P ≤ 0.005 as evidence of association given that we tested nine variants in total. Upon identification of the most T1D-associated variant, we investigated the correlation between its genotype and SLC11A1 expression overall or with splice isoform ratio using 42 PAXgene whole blood samples from healthy donors by quantitative PCR (qPCR). Results Using the case-control collection, rs3731865 (INT4) was identified to be the variant most associated with T1D (P = 1.55 × 10-6). There was also some evidence of association at rs4674297 (P = 1.57 × 10-4). No evidence of disease association was obtained at any of the loci using the family collections (P TDT ≥ 0.13). We also did not observe a correlation between rs3731865 genotypes and SLC11A1 expression overall or with splice isoform expression. Conclusion We conclude that rs3731685 (INT4) in the SLC11A1 gene may be associated with T1D susceptibility in the European ancestry population studied. We did not observe a difference in SLC11A1 expression at the RNA level based on the genotypes of rs3731865 in whole blood samples. However, a potential correlation cannot be ruled out in purified cell subsets especially monocytes or macrophages.
dc.languageEnglishen
dc.language.isoen
dc.titleEvidence of association with type 1 diabetes in the SLC11A1 gene regionen
dc.typeArticle
dc.date.updated2011-06-14T10:03:11Z
dc.rights.holderYang et al.; licensee BioMed Central Ltd.
prism.publicationDate2011en
rioxxterms.versionofrecord10.1186/1471-2350-12-59en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2011-04-27en
dc.contributor.orcidDownes, Kate [0000-0003-0366-1579]
dc.contributor.orcidHowson, Joanna [0000-0001-7618-0050]
dc.identifier.eissn1471-2350
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idNational Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (U01DK062418)
pubs.funder-project-idWellcome Trust (076113/C/04/Z)
pubs.funder-project-idWellcome Trust (079895/Z/06/B)
pubs.funder-project-idWellcome Trust (091157/Z/10/B)
pubs.funder-project-idWellcome Trust (061858/Z/00/E)
pubs.funder-project-idBritish Heart Foundation (RG/08/014/24067)


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