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dc.contributor.authorEgger, Borisen
dc.contributor.authorBoone, Jason Qen
dc.contributor.authorStevens, Naomi Ren
dc.contributor.authorBrand, Andreaen
dc.contributor.authorDoe, Chris Qen
dc.date.accessioned2011-06-14T14:31:14Z
dc.date.available2011-06-14T14:31:14Z
dc.date.issued2007-01-05en
dc.identifier.citationNeural Development 2007, 2:1
dc.identifier.issn1749-8104
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237675
dc.description.abstractAbstract Background The choice of a stem cell to divide symmetrically or asymmetrically has profound consequences for development and disease. Unregulated symmetric division promotes tumor formation, whereas inappropriate asymmetric division affects organ morphogenesis. Despite its importance, little is known about how spindle positioning is regulated. In some tissues cell fate appears to dictate the type of cell division, whereas in other tissues it is thought that stochastic variation in spindle position dictates subsequent sibling cell fate. Results Here we investigate the relationship between neural progenitor identity and spindle positioning in the Drosophila optic lobe. We use molecular markers and live imaging to show that there are two populations of progenitors in the optic lobe: symmetrically dividing neuroepithelial cells and asymmetrically dividing neuroblasts. We use genetically marked single cell clones to show that neuroepithelial cells give rise to neuroblasts. To determine if a change in spindle orientation can trigger a neuroepithelial to neuroblast transition, we force neuroepithelial cells to divide along their apical/basal axis by misexpressing Inscuteable. We find that this does not induce neuroblasts, nor does it promote premature neuronal differentiation. Conclusion We show that symmetrically dividing neuroepithelial cells give rise to asymmetrically dividing neuroblasts in the optic lobe, and that regulation of spindle orientation and division symmetry is a consequence of cell type specification, rather than a mechanism for generating cell type diversity.
dc.languageEnglishen
dc.language.isoen
dc.titleRegulation of spindle orientation and neural stem cell fate in the Drosophilaoptic lobeen
dc.typeArticle
dc.date.updated2011-06-14T14:31:14Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderEgger et al.; licensee BioMed Central Ltd.
prism.publicationDate2007en
dcterms.dateAccepted2007-01-05en
rioxxterms.versionofrecord10.1186/1749-8104-2-1en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2007-01-05en
dc.contributor.orcidBrand, Andrea [0000-0002-2089-6954]
dc.identifier.eissn1749-8104
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (G0300072)
pubs.funder-project-idMRC (G0300723B)


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