A genome annotation-driven approach to cloning the human ORFeome
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Collins, John E.
Wright, Charmain L.
Edwards, Carol A.
Davis, Mathew P.
Grinham, James A.
Cole, Charlotte G.
Goward, Melanie E.
Huckle, Elizabeth J.
Beare, David M.
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Collins, J. E., Wright, C. L., Edwards, C. A., Davis, M. P., Grinham, J. A., Cole, C. G., Goward, M. E., et al. (2004). A genome annotation-driven approach to cloning the human ORFeome.
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Abstract We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
This record's URL: http://www.dspace.cam.ac.uk/handle/1810/237690
Rights Holder: Collins et al.; licensee BioMed Central Ltd.