Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval Drosophila
Pulver, Stefan R
MetadataShow full item record
Nair, A., Bate, M., & Pulver, S. R. (2010). Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval Drosophila.
RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.
Abstract Background The development, morphology and genetics of sensory neurons have been extensively studied in Drosophila. Sensory neurons in the body wall of larval Drosophila in particular have been the subject of numerous anatomical studies, however, little is known about the intrinsic electrical properties of larval sensory cells. Findings We performed whole cell patch recordings from an identified peripheral sensory cell, the dorsal bipolar sensory neuron (dbd) and measured voltage-gated ionic currents in 1st instar larvae. Voltage clamp analysis revealed that dbds have a TEA sensitive, non-inactivating I K type potassium current as well as a 4-AP sensitive, inactivating I A type potassium current. dbds also show a voltage-gated calcium current (I Ca ) and a voltage-gated sodium current (I Na ). Conclusions This work provides a first characterization of voltage-activated ionic currents in an identified body-wall sensory neuron in larval Drosophila. Overall, we establish baseline physiology data for future studies aimed at understanding the ionic and genetic basis of sensory neuron function in fruit flies and other model organisms.
Rights Holder: Nair et al.; licensee BioMed Central Ltd.