Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval Drosophila
Nair, Stefan Robert
Bate, Christopher Michael
Pulver, Stefan R
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Nair, S. R., Bate, C. M., & Pulver, S. R. (2010). Characterization of voltage-gated ionic currents in a peripheral sensory neuron in larval Drosophila. https://doi.org/10.1186/1756-0500-3-154
Abstract Background The development, morphology and genetics of sensory neurons have been extensively studied in Drosophila. Sensory neurons in the body wall of larval Drosophila in particular have been the subject of numerous anatomical studies, however, little is known about the intrinsic electrical properties of larval sensory cells. Findings We performed whole cell patch recordings from an identified peripheral sensory cell, the dorsal bipolar sensory neuron (dbd) and measured voltage-gated ionic currents in 1st instar larvae. Voltage clamp analysis revealed that dbds have a TEA sensitive, non-inactivating I K type potassium current as well as a 4-AP sensitive, inactivating I A type potassium current. dbds also show a voltage-gated calcium current (I Ca ) and a voltage-gated sodium current (I Na ). Conclusions This work provides a first characterization of voltage-activated ionic currents in an identified body-wall sensory neuron in larval Drosophila. Overall, we establish baseline physiology data for future studies aimed at understanding the ionic and genetic basis of sensory neuron function in fruit flies and other model organisms.
External DOI: https://doi.org/10.1186/1756-0500-3-154
This record's URL: http://www.dspace.cam.ac.uk/handle/1810/237825
Rights Holder: Nair et al.; licensee BioMed Central Ltd.