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dc.contributor.authorMcEwen, Kirsten Ren
dc.contributor.authorFerguson-Smith, Anne Carlaen
dc.date.accessioned2011-06-16T16:10:40Z
dc.date.available2011-06-16T16:10:40Z
dc.date.issued2010-01-15en
dc.identifier.citationEpigenetics & Chromatin 2010, 3:2
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237879
dc.description.abstractAbstract Background The field of epigenetics is developing rapidly, however we are only beginning to comprehend the complexity of its influence on gene regulation. Using genomic imprinting as a model we examine epigenetic profiles associated with different forms of gene regulation. Imprinting refers to the expression of a gene from only one of the chromosome homologues in a parental-origin-specific manner. This is dependent on heritable germline epigenetic control at a cis-acting imprinting control region that influences local epigenetic states. Epigenetic modifications associated with imprinting regulation can be compared to those associated with the more canonical developmental regulation, important for processes such as differentiation and tissue specificity. Here we test the hypothesis that these two mechanisms are associated with different histone modification enrichment patterns. Results Using high-throughput data extraction with subsequent analysis, we have found that particular histone modifications are more likely to be associated with either imprinting repression or developmental repression of imprinted genes. H3K9me3 and H4K20me3 are together enriched at imprinted genes with differentially methylated promoters and do not show a correlation with developmental regulation. H3K27me3 and H3K4me3, however, are more often associated with developmental regulation. We find that imprinted genes are subject to developmental regulation through bivalency with H3K4me3 and H3K27me3 enrichment on the same allele. Furthermore, a specific tri-mark signature comprising H3K4me3, H3K9me3 and H4K20me3 has been identified at all imprinting control regions. Conclusion A large amount of data is produced from whole-genome expression and epigenetic profiling studies of cellular material. We have shown that such publicly available data can be mined and analysed in order to generate novel findings for categories of genes or regulatory elements. Comparing two types of gene regulation, imprinting and developmental, our results suggest that different histone modifications associate with these distinct processes. This form of analysis is therefore a useful tool to elucidate the complex epigenetic code associated with genome function and to determine the underlying features conferring epigenetic states.
dc.languageEnglishen
dc.language.isoen
dc.titleDistinguishing epigenetic marks of developmental and imprinting regulationen
dc.typeArticle
dc.date.updated2011-06-16T16:10:40Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderMcEwen et al.; licensee BioMed Central Ltd.
prism.publicationDate2010en
dcterms.dateAccepted2010-01-15en
rioxxterms.versionofrecord10.1186/1756-8935-3-2en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2010-01-15en
dc.contributor.orcidFerguson-Smith, Anne Carla [0000-0003-4996-9990]
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (G0800784)


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