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dc.contributor.authorKoch, Peter
dc.contributor.authorLampe, Marko
dc.contributor.authorGodinez, William J.
dc.contributor.authorMuller, Barbara
dc.contributor.authorRohr, Karl
dc.contributor.authorKraeusslich, Hans-Georg
dc.contributor.authorLehmann, Maik J.
dc.date.accessioned2011-06-16T16:14:20Z
dc.date.available2011-06-16T16:14:20Z
dc.date.issued2009-09-18
dc.identifierhttp://dx.doi.org/10.1186/1742-4690-6-84
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237905
dc.descriptionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.
dc.description.abstractAbstract Background Most retroviruses enter their host cells by fusing the viral envelope with the plasma membrane. Although the protein machinery promoting fusion has been characterized extensively, the dynamics of the process are largely unknown. Results We generated human immunodeficiency virus-1 (HIV-1) particles pseudotyped with the envelope (Env) protein of ecotropic murine leukemia virus eMLV to study retrovirus entry at the plasma membrane using live-cell microscopy. This Env protein mediates highly efficient pH independent fusion at the cell surface and can be functionally tagged with a fluorescent protein. To detect fusion events, double labeled particles carrying one fluorophor in Env and the other in the matrix (MA) domain of Gag were generated and characterized. Fusion events were defined as loss of Env signal after virus-cell contact. Single particle tracking of >20,000 individual traces in two color channels recorded 28 events of color separation, where particles lost the Env protein, with the MA layer remaining stable at least for a short period. Fourty-five events were detected where both colors were lost simultaneously. Importantly, the first type of event was never observed when particles were pseudotyped with a non-fusogenic Env. Conclusion These results reveal rapid retroviral fusion at the plasma membrane and permit studies of the immediate post-fusion events.
dc.language.isoen
dc.rightsAll Rights Reserveden
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/en
dc.titleVisualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy
dc.typeArticle
dc.date.updated2011-06-16T16:14:20Z
dc.description.versionPublished version
dc.rights.holderKoch et al.; licensee BioMed Central Ltd.
pubs.declined2017-10-11T13:54:29.268+0100


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