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dc.contributor.authorHerath, Shanen
dc.contributor.authorLilly, Sonia Ten
dc.contributor.authorSantos, Natalia Ren
dc.contributor.authorGilbert, Robert Oen
dc.contributor.authorGoetze, Leopolden
dc.contributor.authorBryant, Clareen
dc.contributor.authorWhite, John Oen
dc.contributor.authorCronin, Jamesen
dc.contributor.authorSheldon, I Martinen
dc.date.accessioned2011-06-16T16:16:05Z
dc.date.available2011-06-16T16:16:05Z
dc.date.issued2009-05-29en
dc.identifier.citationReproductive Biology and Endocrinology 2009, 7:55
dc.identifier.issn1477-7827
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237915
dc.description.abstractAbstract Background Contamination of the uterine lumen with bacteria is ubiquitous in cattle after parturition. Some animals develop endometritis and have reduced fertility but others have no uterine disease and readily conceive. The present study tested the hypothesis that postpartum cattle that develop persistent endometritis and infertility are unable to limit the inflammatory response to uterine bacterial infection. Methods Endometrial biopsies were collected several times during the postpartum period from animals that were subsequently infertile with persistent endometritis (n = 4) or had no clinical disease and conceived to first insemination (n = 4). Quantitative PCR was used to determine the expression of candidate genes in the endometrial biopsies, including the Toll-like receptor (TLR 1 to 10) family of innate immune receptors, inflammatory mediators and their cognate receptors. Selected proteins were examined by immunohistochemistry. Results The expression of genes encoding pro-inflammatory mediators such as interleukins (IL1A, IL1B and IL6), and nitric oxide synthase 2 (NOS2) were higher during the first week post partum than subsequently. During the first week post partum, there was higher gene expression in infertile than fertile animals of TLR4, the receptor for bacterial lipopolysaccharide, and the pro-inflammatory cytokines IL1A and IL1B, and their receptor IL1R2. The expression of genes encoding other Toll-like receptors, transforming growth factor beta receptor 1 (TGFBR1) or prostaglandin E2 receptors (PTGER2 and PTGER4) did not differ significantly between the animal groups. Gene expression did not differ significantly between infertile and fertile animals after the first week postpartum. However, there were higher ratios of IL1A or IL1B mRNA to the anti-inflammatory cytokine IL10, during the first week post partum in the infertile than fertile animals, and the protein products of these genes were mainly localised to the epithelium of the endometrium. Conclusion Cattle may maintain fertility by limiting the inflammatory response to postpartum bacterial infection in the endometrium during the first week after parturition.
dc.languageEnglishen
dc.language.isoen
dc.titleExpression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertilityen
dc.typeArticle
dc.date.updated2011-06-16T16:16:05Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderHerath et al.; licensee BioMed Central Ltd.
prism.publicationDate2009en
dcterms.dateAccepted2009-05-29en
rioxxterms.versionofrecord10.1186/1477-7827-7-55en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2009-05-29en
dc.contributor.orcidBryant, Clare [0000-0002-2924-0038]
dc.identifier.eissn1477-7827
rioxxterms.typeJournal Article/Reviewen


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