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dc.contributor.authorChuva, de Sousa Lopes Susana Men
dc.contributor.authorHayashi, Katsuhikoen
dc.contributor.authorSurani, Azimen
dc.date.accessioned2011-06-16T16:31:19Z
dc.date.available2011-06-16T16:31:19Z
dc.date.issued2007-12-20en
dc.identifier.citationBMC Developmental Biology 2007, 7:140
dc.identifier.issn1471-213X
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237995
dc.description.abstractAbstract Background The extraembryonic tissues, visceral endoderm (VE) and extraembryonic ectoderm (ExE) are known to be important for the induction of primordial germ cells (PGCs) in mice via activation of the bone morphogenetic protein (BMP) signalling pathway. We investigated whether the VE and ExE have a direct role in the specification of PGCs, or in an earlier event, namely the induction of the PGC precursors in the proximal posterior epiblast cells. Results We cultured embryonic day (E) 5.75 to E7.0 mouse embryos in an explant-assay with or without extraembryonic tissues. The reconstituted pieces of embryonic and extraembryonic tissues were assessed for the formation of both PGC precursors and specified PGCs. For this, Blimp1:gfp and Stella:gfp transgenic mouse lines were used to distinguish between PGC precursors and specified PGC, respectively. We observed that the VE regulates formation of an appropriate number of PGC precursors between E6.25–E7.25, but it is not essential for the subsequent specification of PGCs from the precursor cells. Furthermore, we show that the ExE has a different role from that of the VE, which is to restrict localization of PGC precursors to the posterior part of the embryo. Conclusion We show that the VE and ExE have distinct roles in the induction of PGC precursors, namely the formation of a normal number of PGC precursors, and their appropriate localization during early development. However, these tissues do not have a direct role during the final stages of specification of the founder population of PGCs.
dc.languageEnglishen
dc.language.isoen
dc.titleProximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursorsen
dc.typeArticle
dc.date.updated2011-06-16T16:31:19Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderChuva de Sousa Lopes et al.; licensee BioMed Central Ltd.
prism.publicationDate2007en
dcterms.dateAccepted2007-12-20en
rioxxterms.versionofrecord10.1186/1471-213X-7-140en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2007-12-20en
dc.contributor.orcidSurani, Azim [0000-0002-8640-4318]
dc.identifier.eissn1471-213X
rioxxterms.typeJournal Article/Reviewen


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