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dc.contributor.authorDong, Yanen
dc.contributor.authorBogdanova, Alionaen
dc.contributor.authorHabermann, Biancaen
dc.contributor.authorZachariae, Wolfgangen
dc.contributor.authorAhringer, Julieen
dc.date.accessioned2011-06-16T16:44:19Z
dc.date.available2011-06-16T16:44:19Z
dc.date.issued2007-03-20en
dc.identifier.citationBMC Developmental Biology 2007, 7:19
dc.identifier.issn1471-213X
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/238041
dc.description.abstractAbstract Background RNA interference coupled with videorecording of C. elegans embryos is a powerful method for identifying genes involved in cell division processes. Here we present a functional analysis of the gene B0511.9, previously identified as a candidate cell polarity gene in an RNAi videorecording screen of chromosome I embryonic lethal genes. Results Whereas weak RNAi inhibition of B0511.9 causes embryonic cell polarity defects, strong inhibition causes embryos to arrest in metaphase of meiosis I. The range of defects induced by RNAi of B0511.9 is strikingly similar to those displayed by mutants of anaphase-promoting complex/cyclosome (APC/C) components. Although similarity searches did not reveal any obvious homologue of B0511.9 in the non-redundant protein database, we found that the N-terminus shares a conserved sequence pattern with the N-terminus of the small budding yeast APC/C subunit Cdc26 and its orthologues from a variety of other organisms. Furthermore, we show that B0511.9 robustly complements the temperature-sensitive growth defect of a yeast cdc26Δ mutant. Conclusion These data demonstrate that B0511.9 encodes the C. elegans APC/C subunit CDC-26.
dc.languageEnglishen
dc.language.isoen
dc.titleIdentification of the C. elegansanaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeasten
dc.typeArticle
dc.date.updated2011-06-16T16:44:19Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderDong et al.; licensee BioMed Central Ltd.
prism.publicationDate2007en
dcterms.dateAccepted2007-03-20en
rioxxterms.versionofrecord10.1186/1471-213X-7-19en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2007-03-20en
dc.contributor.orcidAhringer, Julie [0000-0002-7074-4051]
dc.identifier.eissn1471-213X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (054523/Z/98/C)


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